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冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆

DOI: 10.7668/hbnxb.2011.05.008, PP. 34-39

Keywords: 毛百合,冷诱导,甘油-3-磷酸酰基转移酶(GPAT),简并引物,保守区,同源性

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Abstract:

探讨了野生毛百合甘油-3-磷酸酰基转移酶活性在冷胁迫下的变化,用简并引物扩增了甘油-3-磷酸酰基转移酶基因保守区序列的结果表明,该保守区段长744bp,推测编码247个氨基酸。在GPAT氨基酸序列中存在1个高度保守的区域(WIAPSGGRDRP),在NCBI上经过BLASTp比对分析,发现这段保守区序列为LPLAT基因超级家族酶类的催化活性区,此家族多为催化酰基辅酶A(acylCoAs)或者酰基载体蛋白(acylACPs)中的酰基与受体蛋白结合的酰基转移酶类。此序列Genbank登录号为HQ654523。

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