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小麦NBS类抗病基因类似序列的多样性和进化关系研究

DOI: 10.7668/hbnxb.2011.04.003, PP. 23-26

Keywords: 小麦,NBS,抗病基因类似物

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Abstract:

利用已克隆植物抗病基因NBS(Nucleotidebindingsite)序列中的保守结构P-loop和GLPL合成简并引物,以小麦近等基因系TcLr24基因组DNA为模板进行PCR扩增。得到13条具有连续ORF的抗病基因类似物(Resistancegeneanalogues,RGAs)序列,它们之间相应推测的氨基酸序列间的相似性系数在36.9%~98.3%之间。对甘薯RGAs和4个已克隆植物NBS的氨基酸序列进行结构分析表明,它们包括P-loop、Kinase-2、Kinase-3a、GLPL抗病基因所共有的保守结构。同时对分离的RGAs的氨基酸序列进行系统发育树分析,这些RGAs与Xa1、RPS2聚在一起,并且符合nonTIRRGAs类型。这些结果表明小麦与其他物种的NBS类RGAs可能具有同样的起源和进化机制。

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