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抗水稻黑条矮缩病RNA干扰载体的构建及遗传转化

DOI: 10.3969/j.issn.1000-7091.2012.04.027, PP. 144-148

Keywords: 水稻黑条矮缩病,RNA干扰载体,Ubi启动子,bar基因,抗病水稻新品种培育

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Abstract:

水稻黑条矮缩病是由水稻黑条矮缩病毒引起的水稻病害,该病可导致病株生长迟缓、矮化、抽穗结实困难,严重影响了水稻的生产。在水稻黑条矮缩病毒基因组不同双链RNA序列的基础上,分别设计了5对特异性引物,通过RT—PCR获得了相应片段,结合基因沉默的方法,分别构建RNA干扰载体,并进一步将发夹结构构建入以玉米泛素Ubi为启动子、以bar基因为选择标记的改造后的植物双元表达载体pCA1300-Ubi中,经农杆菌介导对黄淮稻区主栽水稻品种圣稻13进行遗传转化,成功获得了5个含有水稻黑条矮缩病干扰片段的植物双元表达载体,经农杆菌介导获得了部分水稻转化植株。旨在为下一步对水稻黑条矮缩病的研究及抗病水稻新品种的培育提供材料。

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