全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

小麦RIL群体第一部分同源群染色体遗传多样性与偏分离分析

DOI: 10.3969/j.issn.1000-7091.2012.04.013, PP. 73-79

Keywords: 小麦,RILs群体,SSR,遗传多样性,偏分离

Full-Text   Cite this paper   Add to My Lib

Abstract:

为了明确小麦第一同源群染色体的遗传多样性,用小麦重组近交系群体(RIL)(Q9086×陇鉴19)108个株系为材料,利用SSR标记对小麦第一同源群进行遗传多样性和偏分离分析。结果表明,97对SSR引物在RIL群体亲本间筛选具有多态性标记23对,多态性频率1B(30.30%)〉1A(28.57%)〉1D(10.34%)。在RIL群体中检出107个等位位点有多态性,每个引物可扩增出2~10个位点,平均4.65个。每个位点多态信息含量(PIC)在0.326—0.880之间,PIC值1B(0.709)〉lA(0.534)〉1D(0.498)。株系间遗传相似系数(GS)在0.40—0.96之间。通过聚类可将RIL群体分为九大类。亲本基因型在群体中的分离比例为1:1.13。通过X^2检测,有15个SSR标记表现为偏分离(P〈0.05),偏分离频率为65.2%,其中,有7个标记偏向父本陇鉴19,8个标记偏向母本Q9086;6个标记偏分离在1A上,7个偏分离在1B上,2个分布在2D上。

References

[1]  Wu X,Wang Z, Chang X,et al. Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes[J]. Journal of Experimental Bota- ny,2010,61(11) :2923 -2937.
[2]  《普通小麦B基因组的研究进展》[J].分子植物育种,2008(4).
[3]  Zhang L,Sun G,Yan Z,et al. Comparison of newly syn- thetic hexaploid wheat with its donors on SSR products [J]. Journal of Genetics and Genomics, 2007,34 ( 10 ) : 939 -946.
[4]  Somers D J, Isaac P, Edwards K. A high-density wheat mi- crosatellite consensus map for bread wheat( Triticum aesti- vum L. ) [J]. Theoretical and Applied Genetics, 2004, 109:1105 - 1114.
[5]  Quarrie S A, Steed A, Calestani C, et al. A high-density genetic map of hexaploid wheat ( Triticum aestivum L. ) from the cross Chinese Spring × SQ1 and its use to com- pare QTLs for grain yield across a range of environments [J]. Theoretical and Applied Genetics, 2005,110 : 865 - 880.
[6]  Su J, Zheng Q, Li H, et al. Detection of QTLs for phos- phorus use efficiency in relation to agronomic perform- ance of wheat grown under phosphorus sufficient and limited conditions [J]. Plant Science, 2009,176 : 824 - 836.
[7]  Yang D, Jing R, Chang X, et al. Identification of quanti- tative trait loci and environmental interactions for accu- mulation and remobilization of water-soluble carbohy- drates in wheat( Triticum aestivum L. ) stems[J]. Genet- ics,2007,176:571 - 584.
[8]  Yang D, Jing R, Chang X, et al. Quantitative trait loci mapping for chlorophyll fluorescence and associated traits in wheat ( Triticum aestivum L. ) [J]. Journal of Integrative Plant Biology,2007,49 ( 5 ) :646 - 654.AA29
[9]  Li H,Tong Y, Li B,et al. Genetic analysis of tolerance to photo-oxidative stress induced by high light in winter wheat( Triticum aestivum L. ) [J]. Journal of Genetics and Genomics,2010,37:399 - 412.
[10]  Peng J H, Fahima T, R~der M S,et al. Microsatellite high density mapping of the stripe rust resistance gene YrH52 region on chromsome 1B and evaluation of its marker as- sisted selection in the F2 generation in wild emmer wheat [J]. New Phytologist,2000,146 ( 1 ) : 141 - 154.
[11]  Wang L F,Ma J X,Zhou R H,et al. Molecular tagging of the yellow rust resistance gene Yr10 in common wheat [J]. Euphytica,2002,124 ( 1 ) : 71 - 73.
[12]  Smith J S C,Chin E C L, Shu H,et al. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L. ) :comparisons with data from RFLPs and Pedigree[J]. Theoretical and Applied Genetics, 1997, 95:163 - 173.
[13]  Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants:Present status and future prospects[J]. Plant Molecular Biology,2005, 57:461 -485.
[14]  Liu Z H, Anderson J A, Hu J,et al. A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utihty for detecting quantitative trait loci [J]. Theoretical and Ap- plied Genetics,2005,111:782 - 794.
[15]  Roder M S,Korzun V,Wendehake K,et al. A microsatel- lite map of wheat[J]. Genetics, 1998,149:2007 - 2023.
[16]  Lu H, Romero-Severson J, Bernardo R. Chromosomal re- gions associated with segregation distortion in maize [J]. Theoretical and Applied Genetics,2002,105:622 -628.
[17]  Xu Y,Zhu L, Xiao J,et al. Chromosomal regions associ- ated with segregation distortion of molecular markers in F2,backcross, doubled haploid, and recombinant inbred populations in rice( Oryza sativa L. ) [J]. Molecular Ge- netics and Genomies, 1997,253:535 - 545.
[18]  Matsushita S, Iseki T, Fukuta Y,et al. Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice vari- eties [J]. Euphytica,2003,134:27 - 32.
[19]  Konishi T, Yano Y, Abe K. Geographic distribution of al- leles at the Ga2 locus for segregation distortion in barley [J]. Theoretical and Applied Genetics, 1992,85:419 - 422.
[20]  Blanco A,Bellomo M P,Cenci A,et al. A genetic linkage map of durum wheat[J]. Theoretical and Applied Genet- ics, 1998,97:721 - 728.
[21]  Messmer M M, Keller M, Zanetti S,et al. Genetic linkage map of a wheat spelt cross[J]. Theoretical and Applied Genetics, 1999,98 : 1163 - 1170.
[22]  Paillard S,Schnurbusch T, Winzeler M, et al. Anintegra- tire genetic linkage map of winter wheat ( Triticum aesti- vum L. ) [J]. Theoretical and Applied Genetics, 2003, 107 : 1235 - 1242.
[23]  《小麦RIL群体SSR分子标记偏分离的遗传分析》[J].麦类作物学报,2011(3).

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133