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类猪圆环病毒P1的感染及其分子特征

DOI: 10.7668/hbnxb.2013.05.006, PP. 34-37

Keywords: 类猪圆环病毒P1,PCR,免疫组化,分子特征

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Abstract:

类猪圆环病毒P1是新发现的一种病原,能引起仔猪出现类似猪断奶后多系统衰竭综合征症状,是目前所知的具有最小基因组的病毒。为了解P1感染范围和基因组分子特征,采用PCR方法扩增P1全基因组,克隆测序后,应用生物信息学进行序列同源性比较及遗传进化分析。结果显示,P1已在我国猪场流行,分离株之间的核苷酸序列同源性为99.1%~100%,可分为2个亚分支;同时免疫组化结果也证实了P1在样品中的分布。

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