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大豆中2个编码PR10蛋白新基因的克隆与分析

DOI: 10.3969/j.issn.1000-7091.2013.04.002, PP. 7-12

Keywords: 大豆,PR10蛋白,基因克隆,表达分析

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Abstract:

从抗大豆花叶病毒病(SMV)大豆材料科丰1号中克隆到2个编码PR10蛋白的新基因Glyma09g04530和Glyma15g15590,Glyma09g04530和Glyma15g15590基因的ORF全长均为477bp,编码158个氨基酸。序列比对与系统发生树分析结果表明:Glyma09g04530和Glyma15g15590是大豆中2个编码PR10蛋白的新基因。Glyma09g04530和Glyma15g15590基因在大豆的根、茎及叶中均有表达;接种大豆SMV后,Glyma09g04530和Glyma15g15590基因在大豆科丰1号的叶片中的表达均被强烈诱导并高效表达,显示Glyma09g04530和Glyma15g15590基因与大豆SMV抗性密切相关。

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