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日粮精粗比对围产期奶牛乳腺内参基因表达的影响

DOI: 10.3969/j.issn.1000-7091.2013.04.014, PP. 75-78

Keywords: 围产奶牛,乳腺,内参基因,实时荧光定量PCR,geNorm程序

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Abstract:

选择合适的内参基因是利用实时荧光定量PCR准确分析基因相对表达量的先决条件。选择了奶牛乳腺研究中广泛使用的7个候选内参基因,包括GAPDH、ACTB、18SrRNA、RPS9、RPS15、UXT、MYC。奶牛以精粗比不同的2种日粮饲喂,并在产后16d采集乳腺组织。利用qRT-PCR技术探讨了候选内参基因在围产奶牛乳腺组织中的表达情况,并利用geNorm程序对数据进行分析。结果发现,UXT和RPS9表达稳定,不受日粮精粗比的影响,因此,可作为围产期奶牛乳腺组织基因表达分析中相对合适的内参基因。

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