全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化

DOI: 10.3969/j.issn.1000-7091.2013.03.011, PP. 58-61

Keywords: TaNADP-ME2,农杆菌,基因转化,水稻

Full-Text   Cite this paper   Add to My Lib

Abstract:

小麦TaNADP-ME2是一光反应基因,并对干旱、盐、低温等非生物胁迫作出响应。构建TaNADP-ME2基因的重组植物表达载体,并转化水稻获得转基因植株。利用重组技术构建植物表达载体,冻融法转化农杆菌,农杆菌介导方法转化水稻成熟胚诱导的愈伤组织,潮霉素筛选和PCR鉴定转基因植株。成功构建了重组植物表达质粒pSUE2,并将pSUE2转入农杆菌EHA105,潮霉素磷酸转移酶(HPT)基因和TaNADP-ME2基因PCR鉴定获得16棵转基因阳性水稻植株。为进一步确定TaNADP-ME2基因的水分利用效率功能奠定了基础。

References

[1]  张正斌,徐萍,周晓果,等. 作物水分利用效率的遗传改良研究进展[J]. 中国农业科学, 2006, 39( 2) : 289 -294.
[2]  Zhu Y L,Lin K R,Huang Y H, et al. A cDNA from Tomato( Lycope rsicon pennellii) encoding ribulose-1,5-bisphosphate carboxylase /oxygenase activase [J]. Plant Physiology, 1998, 116: 1603.
[3]  Torii K U,Mitsukawa N,Oosuml T, et al. The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats [J]. The Plant Cell, 1996,8: 735 - 746.
[4]  Jorda L,Molina A. ERECTA receptor-like kinase and heterotrimeric G protein from Arabidopsis are required for resistance to the necrotrophic fungus plectosphaerella cucumerina[J]. Plant Journal, 2005, 43( 2) : 165 - 180.
[5]  Masle J,Gilmore S R,Farquhar G D. The ERECTA gene regulates plant transpiration efficiency in Arabidopsis[J].Nature, 2005, 436: 866 - 870.
[6]  Karaba A,Dixit S,Greco R, et al. Improvement of water use efficiency in rice by expression of HARDY, an Arabidopsis drought and salt tolerance gene[J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104: 15270 - 15275.
[7]  Sade N,Gebretsadik M,Seligmann R, et al. The role of tobacco aquaporin1 in improving water use efficiency,hydraulic conductivity, and yield production under salt stress[J]. Plant Physiology, 2010, 152: 245 - 254.
[8]  Shearer H L,Turpin D H,Dennis D T. Characterization of NADP-dependent malic enzyme from developing castor oil seed endosperm[J]. Archives of Biochemistry and Biophysics, 2004, 429: 134 - 144.
[9]  Chi W,Yang J H,Wu N H, et al. Four rice genes encoding NADP-malic enzyme exhibit distinct expression profiles[J]. Bioscience Biotechnology and Biochemistry,2004, 68: 1865 - 1874.
[10]  Ferreyra M L F,Andreo C S,Podestá F E. Purification and physical and kinetic characterization of a photosynthetic NADP-dependent malic enzyme from the CAM plant Aptenia cordifolia[J]. Plant Science,2003,164:95 - 102.
[11]  Fu Z Y,Zhang Z B,Hu X J, et al. Cloning, identification,expression analysis and phylogenetic relevance of two NADP-dependent malic enzyme genes from hexaploid wheat[J]. Comptes Rendus Biologies,2009,332:591 - 602.
[12]  Fu Z Y,Zhang Z B,Liu Z H, et al. Effects of different abiotic stresses on hexaploid wheat ( Triticum aestivum L. ) NADP-dependent malic enzyme ( NADP-ME ) [J]. Biologia Plantarum, 2011, 55: 196 - 200.
[13]  殷奎德,张兴梅,郑桂萍,等. 一种快速检测转基因水稻潮霉素抗性标记的方法[J]. 黑龙江八一农垦大学学报, 2010, 22( 1) : 45 - 48.
[14]  Sivamani E,Bahieldin A,Wraith J M, et al. Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene[J]. Plant Science,2000,155: 1 - 9.
[15]  Drincovich M F,Casati P,Andreo C S. NADP-malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways[J]. FEBS Letters, 2001, 490: 1-6
[16]  Casati P,Drincovich M S,Edwards G E,et al. Malate metabolism by NADP-malic enzyme in plant defense[J]. Photosynthesis Research, 1999, 61: 99 - 105.
[17]  Gerrard Wheeler M C,Arias C L,Tronconi M A,et al.Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties[J].Plant Molecular Biology, 2008, 67: 231 - 242.
[18]  Liu S K,Cheng Y X,Zhang X X,et al. Expression of an NADP-malic enzyme gene in rice ( Oryza sativa L. ) is induced by environmental stresses; over-expression of the gene in Arabidopsis confers salt and osmotic stress tolerance[J]. Plant Molecular Biology, 2007, 64: 49 - 58.
[19]  Cheng Y X,Mei L. A cytosolic NADP-malic enzyme gene from rice ( Oryza sativa L. ) confers salt tolerance in transgenic Arabidopsis[J]. Biotechnology Letters,2007, 29: 1129 - 1134.
[20]  Laporte M M,Shen B,Tarczynski M C. Engineering for drought avoidance: expression of maize NADP-malic enzyme in tobacco results in altered stomatal function[J]. Journal of Experimental Botany, 2002, 53( 369) : 699 -705.
[21]  Detarsio E,Maurino V G,Alvarez C E, et al. Maize cytosolic NADP-malic enzyme ( ZmCytNADP-ME) : a phylogenetically distant isoform specifically expressed in embryo and emerging roots[J]. Plant Molecular Biology,2008, 68: 355 - 367.
[22]  Müller G L,Drincovich M F,Andreo C S,et al. Nicotiana tabacum NADP-malic enzyme: cloning, characterization and analysis of biological role[J]. Plant and Cell Physiology, 2008, 49: 469 - 480.
[23]  Gerrard Wheeler M C,Tronconi M A,Drincovich M F, et al. A comprehensive analysis of the NADP-malic enzyme gene family of Arabidopsis thaliana[J]. Plant Physiology,2005, 139: 39 - 51.
[24]  Saigo M,Bologna F P,Maurino V G,et al. Maize recombinant non-C4 NADP-malic enzyme: A novel dimeric malic enzyme with high specific activity[J]. Plant Molecular Biology,2004, 55: 97 - 107.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133