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泌盐植物长叶红砂质膜Na+/H+逆向转运蛋白基因(RtSOS1)全长cDNA的克隆及序列分析

DOI: 10.3969/j.issn.1000-7091.2013.03.001, PP. 1-6

Keywords: 长叶红砂,质膜Na+/H+逆向转运蛋白基因,全长cDNA克隆

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Abstract:

长叶红砂为内蒙古东阿拉善-西鄂尔多斯地区特有珍稀泌盐,强旱生小灌木,对盐渍荒漠环境具有极强适应性。利用RT-PCR和RACE技术从该植物中分离出质膜Na+/H+逆向转运蛋白基因(RtSOS1),该cDNA全长为3829bp,开放阅读框为3438bp,编码一个含1145个氨基酸的蛋白质,推测分子量为126.76kDa。氨基酸序列的生物信息学分析推测,该蛋白N端含有11个跨膜结构域,C端为一个长约700个氨基酸的亲水性尾,具有磷酸化和自我抑制结构域。同源性比对和系统发育分析证实,RtSOS1与其他植物的质膜Na+/H+逆向转运蛋白亲缘关系较近。

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