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利用STS标记检测我国小麦推广品种的抗穗发芽基因型

DOI: 10.3969/j.issn.1000-7091.2013.03.033, PP. 183-188

Keywords: 小麦,穗发芽,分子标记,Vp1A3,Vp1B3

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Abstract:

利用已开发的与穗发芽抗性相关的STS标记Vp1A3和Vp1B3,对我国46份代表性小麦品种进行穗发芽抗性基因的综合筛选。结果表明:用Vp1A3共检测出13种Vp-1A等位基因,分别是Vp-1Aam、Vp-1Aan、Vp-1Aao、Vp-1Abm、Vp-1Abn、Vp-1Agm、Vp-1Agn、Vp-1Ahm、Vp-1Aho、Vp-1Aim、Vp-1Ain、Vp-1Aio、Vp-1Ajm,各占总数的17.4%,8.7%,2.2%,4.3%,4.3%,6.5%,2.2%,2.2%,2.2%,15.2%,28.3%,4.3%,2.2%;用Vp1B3共检测出3种Vp-1B的等位基因,分别为Vp-1Bc、Vp-1Ba、Vp-1Bb,各占总数的71.7%,17.4%,4.3%。通过Vp1A3和Vp1B3的综合筛选,在46份材料中共选出5份抗穗发芽基因型材料,分别是绵阳31、豫麦35、凤麦24、CA0489和山东928802,其Vp-1基因的单倍型分别为:Vp-1Agm和Vp-1Bc,Vp-1Agm和Vp-1Bc,Vp-1Agm和Vp-1Bc,Vp-1Aam和Vp-1Bb,Vp-1Abm和Vp-1Bb。利用Vp1A3和Vp1B3对小麦的穗发芽抗性基因进行综合筛选,提高了选择效率。

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