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百脉根S-腺苷甲硫氨酸脱羧酶基因克隆与表达分析

DOI: 10.3969/j.issn.1000-7091.2013.02.015, PP. 78-85

Keywords: 百脉根,多胺,S-腺苷甲硫氨酸脱羧酶,LcSAMDC1

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Abstract:

S-腺苷甲硫氨酸脱羧酶(SAMDC|EC4.1.1.50)是多胺合成过程中的限速酶。以日本百脉根EST序列为基础,通过设计特异引物进行RT-PCR扩增,获得了S-腺苷甲硫氨酸脱羧酶编码cDNA,命名为LcSAMDC1。LcSAMDC1基因组序列全长2626bp,存在3个内含子|cDNA序列全长1799bp,存在3个植物SAMDC基因特征性ORF,其中tORF编码2个氨基酸残基,sORF编码55个氨基酸残基,mORF编码354个氨基酸残基|mORF编码产物推断的分子量为38.6kDa,具有2个高度保守的功能结构域(酶原剪切位点和PEST结构域)|半定量RT-PCR分析显示,LcSAM-DC1在幼叶和根中具有高水平的表达,在茎中表达水平较低,而在成熟叶片中不表达。

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