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不同谷物籽粒木聚糖酶抑制蛋白活性的分布特征研究

DOI: 10.3969/j.issn.1000-7091.2013.01.026, PP. 140-143

Keywords: 谷物,籽粒,木聚糖酶抑制蛋白,活性,分布

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Abstract:

为准确了解谷物中木聚糖酶抑制蛋白活性的存在情况,从而进一步分析这种抑制蛋白活性对谷物加工中外加木聚糖酶可能产生的负面影响。选取小麦、大麦、黑麦、绿粒小麦、荞麦、燕麦、玉米、水稻和高粱9种常见的谷物为材料,测定了其籽粒以及籽粒不同部位木聚糖酶抑制蛋白的活性。结果表明,木聚糖酶抑制蛋白活性在小麦、黑麦、绿麦和荞麦中较高,分别为4.27,4.76,4.18,3.61IA/mg;在大麦、燕麦中较低,分别为0.54,0.87IA/mg;玉米、水稻和高粱中没有发现木聚糖酶抑制蛋白活性。进一步选取木聚糖酶抑制蛋白活性较高的小麦、黑麦和绿粒小麦,分别测定其种皮、胚和胚乳中的木聚糖酶抑制蛋白活性。结果显示,小麦种皮、胚和胚乳中的木聚糖酶抑制蛋白活性分别是0.06,3.67,4.47IA/mg;黑麦种皮、胚和胚乳中的木聚糖酶抑制蛋白活性分别是1.70,5.42,5.39IA/mg;绿粒小麦种皮、胚和胚乳中的木聚糖酶抑制蛋白活性分别是0,5.06,4.57IA/mg。3种麦类表现出一致的结果,即木聚糖酶抑制蛋白活性在胚和胚乳中基本持平,并且远高于种皮。

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