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GFLVCP基因RNAi载体构建及其在转基因烟草中的干涉效果

DOI: 10.7668/hbnxb.2014.S1.001, PP. 1-5

Keywords: 葡萄扇叶病毒,RNAi,载体构建,转基因,烟草

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Abstract:

为探讨RNAi策略在抗GFLV基因工程中的效果,通过RT-PCR克隆获得了310bp的GFLV外壳蛋白基因保守片段,采用Gateway技术将该基因片段连入目标载体pHELLSGATE12构建了RNAi植物表达载体,并通过农杆菌介导法转入烟草中,RT-PCR检测表明目的基因片段已整合到烟草基因组中并在RNA水平上得到表达,病毒接种结果显示阳性植株的发病率明显低于对照,说明在转基因植株中dsRNA表达可干涉GFLV侵染过程。

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