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乌鲁木齐三角荨麻白粉病菌鉴定和进化分析

DOI: 10.7668/hbnxb.2014.06.025, PP. 145-148

Keywords: 三角荨麻,白粉病菌,鉴定,进化分析

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Abstract:

通过显微观察和核糖体DNA转录间隔区(ITS)序列分析对引起乌鲁木齐地区三角荨麻白粉病的病原进行了鉴定。病原菌的分生孢子梗顶端产生一个椭圆形的分生孢子,分生孢子(18.75~23.75)μm×(31.25~43.75)μm;黑褐色、球形的闭囊壳嵌在叶表面的菌丝体中,直径为87.5~143.75μm;每个闭囊壳中有6或8个子囊,子囊无柄,大小为(25~43.75)μm×(37.5~68.75)μm,每个子囊中有4或5个子囊孢子,每个子囊孢子(18.75~21.88)μm×(25~31.25)μm。采用真菌ITS通用引物,扩增病菌rDNAITS,测序及进化分析后发现,其ITS序列与伊朗异株荨麻白粉病菌(登录号AB104524)的同源性为99%,而且聚在一个进化支上。结果表明,乌鲁木齐三角荨麻白粉病菌属于Erysipheurticae,与伊朗异株荨麻白粉病菌亲缘关系最近。

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