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小麦三雌蕊突变体TaAP1-3基因的克隆及表达分析

DOI: 10.7668/hbnxb.2014.06.006, PP. 28-35

Keywords: 小麦,花器官发育,TaAP1-3,MADS-box基因,基因表达

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Abstract:

为探讨TaAP1-3基因在小麦三雌蕊性状形成中的作用,从小麦三雌蕊近等基因系CM28和CM28TP中克隆得到3个同源基因TaAP1-3a、TaAP1-3b和TaAP1-3c。通过碱基序列、氨基酸序列、聚类及实时荧光定量分析表明,TaAP1-3a、TaAP1-3b和TaAP1-3c基因cDNA序列分别为1210,1208,1199bp,ORF分别为825,816,855bp,分别编码274,271,284个氨基酸残基。TaAP1-3a、TaAP1-3b和TaAP1-3c与中国春TaAP1-3的核苷酸序列相似度分别为96.02%,93.1%,93.56%,氨基酸序列相似性高达99%,95%,97%,并且与A类基因TaAGL29、OsMADS15、ZAP1、BM8、EnWM8、TaAP1-3聚集到AP1/SUQA亚家族FUL2类群中。实时荧光定量分析表明,TaAP1-3a、TaAP1-3b和TaAP1-3c在CM28和CM28TP的3个发育时期的小穗中均有表达,但各个时期的表达量有明显差异。CM28中主要在二棱期-小花分化期表达,CM28TP中主要在雌雄蕊原基分化期表达。试验分析显示,TaAP1-3a、TaAP1-3b和TaAP1-3c可能具有上述A类基因相似的功能,其表达模式可能与小麦三粒/一粒性状形成相关。试验结果为进一步探讨该基因在小麦三雌蕊性状形成过程中的作用奠定了基础。

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