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转盐地碱蓬SsDREB基因烟草的抗逆生理机制分析

DOI: 10.7668/hbnxb.2014.06.027, PP. 155-162

Keywords: 烟草,SsDREB基因,转基因,抗逆性,生理指标

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Abstract:

为了研究盐地碱蓬SsDREB基因在转基因烟草中的功能,探究转基因烟草的抗逆生理机制,我们构建了SsDREB基因的植物高效表达载体pCAMBIA2301-SsDREB,并通过农杆菌介导法将其导入烟草NC89中,通过Kan抗性、PCR及RT-PCR筛选鉴定阳性苗;对转基因烟草苗进行高盐、干旱等抗逆性分析,同时测定在不同浓度NaCl和PEG6000处理后转基因烟草叶片的净光合速率(Pn)、气孔导度(Gs)、光能转化效率(Fv/Fm)、实际光量子效率(φPSⅡ)以及脯氨酸和可溶性糖的含量。结果表明:通过Kan抗性、PCR及RT-PCR筛选鉴定,最终获得12个株系转基因阳性苗。转基因植株的抗逆性与对照相比明显提高。在不同浓度NaCl和PEG6000处理下,对照和转基因苗的Pn和Gs随着处理浓度的提高均呈现逐渐下降趋势,但在相同浓度处理下转基因苗的Pn和Gs均比对照高;随着NaCl处理浓度的增大,对照和转基因植株的Fv/Fm和φPSⅡ均逐渐降低,但转基因苗的降低幅度比对照小;随着PEG处理浓度的提高,对照和转基因植株的Fv/Fm逐渐降低,但转基因苗的降低程度较对照小,而φPSⅡ下降趋势基本相同。在不同浓度NaCl和PEG处理后,转基因植株和对照植株脯氨酸和可溶性糖含量均随着NaCl处理浓度的增加而提高,但是转基因植株较对照植株增高更快。

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