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毛尖紫萼藓类泛素蛋白基因的克隆与表达分析

DOI: 10.7668/hbnxb.2014.06.011, PP. 58-62

Keywords: 毛尖紫萼藓,类泛素蛋白基因(Gp-UBL5),基因克隆,实时荧光定量PCR

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Abstract:

为研究类泛素蛋白基因在苔藓植物中的生物学功能,从毛尖紫萼藓干旱cDNA文库中获得了类泛素蛋白基因cDNA全长序列,命名为Gp-UBL5。该基因cDNA全长471bp,包含1个222bp的完整开放阅读框,编码73个氨基酸组成的蛋白。生物信息学分析表明,Gp-UBL5基因编码蛋白分子质量为8.525kDa,等电点为7.84,为稳定型蛋白。进化分析显示,该编码蛋白与二穗短柄草、大麦等类泛素蛋白具有较高一致性,达96%~99%。实时荧光定量PCR结果表明,Gp-UBL5基因在复水和快速干旱的各个时期均有表达,推测该基因可能在毛尖紫萼藓的抗旱机制中发挥作用。

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