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一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发

DOI: 10.7668/hbnxb.2014.06.001, PP. 1-5

Keywords: 大白菜,QTL,TuMV,分子标记

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Abstract:

为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期TuMV-C4抗性相关的主效QTL位点BrTuA09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个InDel在双亲间具有多态性,且条带单一、扩增稳定;连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTuA09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜TuMV抗病分子育种奠定了良好的基础。

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