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文冠果不同花朵类型植株miRNA表达差异分析

DOI: 10.7668/hbnxb.2014.05.004, PP. 16-22

Keywords: 文冠果,miRNA,实时荧光定量PCR,表达分析

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Abstract:

为了探索miRNA在文冠果不同类型植株的花发育阶段不同组织的表达特异性,以单瓣型和重瓣型文冠果的花芽、茎、叶为试材,提取这2种类型组织的小RNA。从前期研究获得的文冠果小RNA测序数据库中筛选11个候选miRNA,采用实时荧光定量PCR法检测其在花发育不同阶段以及茎和叶片的表达特点。结果表明,11个候选miRNA具有不同的表达形式。Xso-M007a-b、miR167a-b与miR169在两类中都是表达量随花芽发育呈现下降趋势,Xso-M003则相反,说明了miRNA表达的时序性。在不同类型之间以及不同部位miRNA的表达也存在差异,Xso-M002、Xso-M003、Xso-M007a-b、miR169、miR319和miR827在两类型花芽中的表达量均高于茎和叶中的表达量。对不同类型、部位和时间点上的表达情况分析,表明miRNA具有明显的品种特异性、组织特异性和时序性。此外,结合miRNA的功能与其表达量差异分析发现,文冠果的形态结构特征与miRNA的表达量变化密切相关。为探索miRNA表达量的变化在林木花器官形成与不同组织分化中的作用提供了重要信息。

References

[1]  Reinhart B J,Weinstein E G,Rhoades M W,et al.MicroRNAs in plants [J].Genes and Development,2002,16(13):1616-1626.
[2]  Jones-Rhoades M W,Bartel D P,Bartel B.MicroRNAs and their regulatory roles in plants[J].Annu Rev Plant Biol,2006,57:19-53.
[3]  Chen X M.MicroRNA biogenesis and function in plants[J].FEBS Letters,2005,579(26,SI):5923-5931.
[4]  陈 新,曾长英,卢 诚,等.基于PCR技术的miRNA定量检测方法[J].中国生物工程杂志,2010,30(11): 88-93.
[5]  Shi R,Chiang V L.Facile means for quantifying microRNA expression by real-time PCR[J].Biotechniques,2005,39(4):519-525.
[6]  魏志刚,钱婷婷,张凯旋,等.外源GA_3对白桦成花基因影响的定量PCR分析[J].林业科学,2011,47(7):187-192.
[7]  杨 帆,丁 菲,杜天真.盐胁迫下构树DREB转录因子基因表达的实时荧光定量PCR分析[J].林业科学,2010,46(4):146-150.
[8]  敖 妍,段 劼,于海燕,等.文冠果研究进展[J].中国农业大学学报,2012,17(6):197-203.
[9]  彭伟秀,李凤兰,王保柱.文冠果同源异型变异株的发现[J].北京林业大学学报,1999,21(5):92-94.
[10]  Ao Y,Wang Y W,Chen L,et al.Identification and comparative profiling of microRNAs in wild-type Xanthoceras sorbifolia and its double flower mutant[J].Genes & Genomics,2012,34(5):561-568.
[11]  Nag A,King S,Jack T.miR319a targeting of TCP4 is critical for petal growth and development in Arabidopsis [J].Proceedings of the National Academy of Sciences of the USA,2009,106(52):22534-22539.
[12]  Nagpal P,Ellis C M,Weber H, et al.Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation [J].Development,2005,132(18):4107-4118.
[13]  Rubio-Somoza I,Weigel D.MicroRNA networks and developmental plasticity in plants [J].Trends Plant Science,2011,16(5):258-264.
[14]  Kasschau K D,Fahlgren N,Chapman E J,et al.Genome-wide profiling and analysis of Arabidopsis siRNAs[J].PLOS Biology,2007,5(3):479-493.
[15]  Rajagopalan R,Vaucheret H,Trejo J,et al.A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana [J].Gene and Development,2006,20(24):3407-3425.
[16]  Nakano M,Nobuta K,Vemaraju K,et al.Plant MPSS databases:signature-based transcriptional resources for analyses of mRNA and small RNA [J].Nucleic Acids Research,2006,34(SI):731-735.
[17]  Jung J H,Park C M.MIR166/165 genes exhibit dynamic expression patterns in regulating shoot apical meristem and floral development in Arabidopsis [J].Planta,2007,225(6):1327-1338.
[18]  Wu M F,Tian Q,Reed J W. Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression,and regulates both female and male reproduction[J].Development,2006,133(21):4211-4218.
[19]  Theissen G.Development of floral organ identity:stories from the MADS house [J].Current Opinion in Plant Biology,2001,4(1):75-85.
[20]  Cartolano M,Castillo R,Efremova N,et al.A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity [J].Nature Genetics,2007,39(7):901-905.
[21]  Hsieh L C,Lin S I,Shih A C,et al.Uncovering small RNA-mediated responses to phosphate deficiency in Arabidopsis by deep sequencing[J].Plant Physiology,2009,151(4):2120-2132.

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