全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

利用双尾法定位大豆水溶性蛋白QTL

DOI: 10.7668/hbnxb.2014.04.013, PP. 80-86

Keywords: 大豆,水溶性蛋白,双尾法,QTL

Full-Text   Cite this paper   Add to My Lib

Abstract:

大豆水溶性蛋白是大豆籽粒蛋白质的主要组成部分,其含量是评价大豆品质及衡量其价值的重要指标。以高水溶性蛋白含量(38.70±1.32)%的冀豆12和正常水溶性蛋白含量(32.40±1.08)%的黑豆(ZDD03651)为亲本构建的包含188个家系的F8、F9重组自交系群体为材料,利用双尾法在3个不同选择压力下对籽粒水溶性蛋白含量进行QTL定位研究。结果表明,2年中重组自交家系群体表型均呈现正态性超双亲分离,在30%,20%,10%这3个不同选择压力下共检测到大豆籽粒水溶性蛋白含量相关的QTL23个,分布在A1、A2、C2、D1b、D2、E、F、H、I、K、L、M和O等13个连锁群中,其中13个QTL增效基因来自冀豆12。2年中在10%的选择压力下同时检测到2个QTL,分别为qSPC-6-1和qSPC-8-1。其中qSPC-6-1的连锁标记为Sat_062,2年中贡献率分别为9.78%和9.66%,增效基因来自冀豆12;qSPC-8-1的连锁标记为Satt177,2年中贡献率分别为9.78%和7.98%,增效基因来自于黑豆。该结果为分子标记辅助育种及分子克隆提供理论依据。

References

[1]  Malhotra A,Coupland J N.The effect of surfactants on the solubility,zeta potential,and viscosity of soy protein isolates[J].Food Hydrocolloids,2004,18(1):101-108.
[2]  Walsh D J,Cleary D,McCarthy E,et al.Modification of the nitrogen solubility properties of soy protein isolate following proteolysis and transglutaminase cross-linking[J].Food Research International,2003,36:677-683.
[3]  Wolf W J.Chemical and physical properties of soybean proteins[J].Bakers Digest,1969,43:30-35.
[4]  Lu W,Wen Z,Li H ,et al. Identification of the quantitative trait loci(QTL)underlying water soluble protein content in soybean[J].Theoretial and Applied Genetics,2013,126(2):425-433.
[5]  Beilinson V,Chen Z,Shoemaker R C,et al.Genomic organization of glycinin genes in soybean[J].Theoretical and Applied Genetics,2002,104(6/7):1132-1140.
[6]  Panthee D R,Kwanyuen P,Sams C E,et al.Quantitative trait loci forβ-conglycinin(7S)and glycinin(11S)fractions of soybean storage protein[J].J Am Oil Chem Soc,2004,81:1005-1012.
[7]  刘顺湖,周瑞宝,喻德跃,等.大豆蛋白质有关性状的QTL定位[J].作物学报,2009,35(12):2139-2149.
[8]  孙艳萍.选择基因型作图方法在数量性状基因定位中的有效性研究[D].北京:中国农业科学院,2010.
[9]  Soufflet-Freslon V,Gianfranceschi L,Patocchi A, et al. Inheritance studies of apple scab resistance and identification of Rvi14,a new major gene that acts together with other broad-spectrum QTL[J].Genome,2008,51(8):657-667.
[10]  Gallais A,Moreau L,Charcosset A.Detection of marker-QTL associations by studying change in marker frequencies with selection[J].Theoretical and Applied Genetics,2007,114(4):669-681.
[11]  Navabi A,Mather D E,Bernier J,et al.QTL detection with bidirectional and unidirectional selective genotyping:marker-based and trait-based analyses[J].Theoretical and Applied Genetics,2009,118(2):347-358.
[12]  Sun Y P,Wang J K,Crouch J H,et al.Efficiency of selective genotyping for genetic analysis of complex traits and potential applications in crop improvement[J].Molecular Breeding,2010,26(3):493-511.
[13]  李卫东,张孟臣.黄淮海夏大豆及品种参数[ M].北京:中国农业科学技术出版社,2006.
[14]  闫龙,冯燕,杨春燕,等.冀豆12遗传背景导入系蛋白、脂肪含量分布特征[J].华北农学报,2012,27(1):87-92.
[15]  陈强,闫龙,杨春燕,等.冀豆12遗传背景下3个回交组合高低蛋白含量后代品系SSR标记分析[J].中国农业科学,2014,47(2):230-239.
[16]  张孟臣,唐晓东.一种大豆水溶性蛋白检测方法[P].中国专利:201110236317.1,2013-7-10.
[17]  Kaufinan B,Richards S,Diefig D A.DNA isolation method for high polysaecharide Lesquuerella species[J].Industrial Crops and Products,1999,9(2):111-114.
[18]  Cregan P B,Jarvik T,Bush A L,et al.An integrated genetic linkage map of the soybean genome [J].Crop Science,1999,39(5):1464-1490.
[19]  Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109(1):122-128.
[20]  王涛,杨春燕,赵青松等.两个大豆开花期QTL定位及对农艺性状的影响分析[J].华北农学报,2013,28(2):63-69.
[21]  Rhee K C.Functionality of soy proteins[A].//Hettiarchchy N A,Ziegler G R(eds)Protein functionality in food systems[C].New York:Marcel Dekker,1994:311-324.
[22]  Thanh V H,Shibasaki K.Major proteins of soybean seeds.A straight forward fractionation and their characterization[J].J Agric Food Chem,1976,24:1117-1121.
[23]  Nandi S,Subudhi P K,Senadhira D,et al.Mapping QTLs for submergence tolerance in rice by AFLP analysis and selective genotyping[J].Mol Gen Genet,1997,255:1-8.
[24]  Merk H L,Ashrafi H,Foolad M R.Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum Pimpinellifolium [J].Euphytica,2012,187(1):63-75.
[25]  Darvasi A,Soller M.Selective genotyping for determination of linkage between a marker locus and a quantitative trait locus[J].Theoretical and Applied Genetics,1992,85(2/3):353-359.
[26]  Hallauer A R,Miranda F.Quantitative genetics in Maize Breeding[M].Ames:Iowa State University Press,1981.
[27]  Falconer D S.Introduction to Quantitative Genetics[M].London:Longman Scientific and Technical,1989.
[28]  Foolad M R,Jones R A.Mapping salt-tolerance genes in tomato(Lycopersicon esculentum)using trait-based marker analysis[J].Theor Appl Genet,1993,87:184-192.
[29]  Foolad M R,Stoltz T,Dervinis C,et al.Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping[J].Mol Breed,1997,3:269-277.
[30]  Zhang L P,Lin G Y,Liu D N,et al.Mapping QTLs conferring early blight(Alternaria solani)resistance in a Lycopersicon esculentum×L.hirsutum cross by selective genotyping[J].Mol Breed,2003,12:3-19.
[31]  McCouchsr S R,Cho Y G,Yano M, et al.Report on QTL nomenclature[J].Rice Genet News Letter,1997,14:11-13.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133