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含GmNHX1和LcVP1双价基因植物表达载体构建与转基因棉花的获得

DOI: 10.7668/hbnxb.2014.04.010, PP. 56-62

Keywords: 棉花,耐盐,大豆液泡膜Na+/H+反向转运蛋白GmNHX1,百脉根液泡膜H+-PPaseLcVP1,遗传转化

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Abstract:

应用DNA重组技术将编码大豆液泡膜Na+/H+反向转运蛋白GmNHX1和百脉根液泡膜H+-PPaseLcVP1的开放阅读框片段同时构建在pCambia1301载体中,形成p1301-GmNHX1-LcVP1双价耐盐基因植物表达载体。以Coker312棉花品种的胚性愈伤组织为外植体,采用根癌农杆菌介导法将其转入棉花中,并利用潮霉素筛选获得抗性植株。对抗性植株的T1幼苗进行分子和生理指标检测,表明外源基因已成功整合到棉花基因组中,并不同程度地提高了转基因株系耐盐能力。

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