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玉米CMS-C不育系及保持系差异基因的克隆与表达分析

DOI: 10.7668/hbnxb.2014.03.003, PP. 11-15

Keywords: 玉米,CMS-C,嵌合ORF,差异表达

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Abstract:

以玉米不育系C48-2及保持系N48-2为材料,利用PCR和RT-PCR技术克隆了嵌合orf118-b并研究了cox2转录本的结构变化。orf118-b是玉米C48-2线粒体基因组特有的嵌合orf,并能在单核期花药中特异表达。cox2*仅在N48-2单核期花药中特异表达,cox2*是cox2基因转录本GRMZM5G862955_T01的新注释,其5'UTR区域被延长。利用Real-TimeqPCR检测了cdpk在不育系及保持系雄穗发育的花粉母细胞时期、四分体时期、单核期和双核期的基因表达水平。结果表明,cdpk在C48-2的母细胞时期、四分体时期、双核期上调表达,在C48-2的单核期下调表达。这些发现为进一步探索玉米CMS-C核质互作导致花粉败育的分子机制提供参考。

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