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IHNV核衣壳蛋白在杆状病毒表达系统中的表达及鉴定

DOI: 10.7668/hbnxb.2014.03.008, PP. 36-40

Keywords: 传染性造血器官坏死病病毒,核衣壳蛋白基因,重组杆状病毒

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Abstract:

采用RT-PCR方法从传染性造血器官坏死病病毒(IHNV)中扩增RNA获得1176bp的核衣壳蛋白(N)基因,将其插入于pFB-LIC-Bse杆状病毒载体中,构建了重组质粒pFB-LIC-Bse-N,转化至感受态细胞DH10Bac中,获得重组杆粒rBacmid-N。再将其转染至Sf9昆虫细胞,获得重组杆状病毒。IFA和WesternBlot分析表明,重组N蛋白可以被辣根过氧化物酶(HRP)标记的组氨酸单抗(anti-His)识别,表明,IHNVN基因在昆虫细胞Sf9中获得了正确表达。为深入研究N蛋白的功能和免疫学特性奠定了基础。

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