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猪圆环病毒2型荧光定量PCR检测方法的建立

DOI: 10.7668/hbnxb.2014.02.012, PP. 66-70

Keywords: 猪圆环病毒2型,SYBR,GreenⅠ,荧光定量PCR

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Abstract:

根据PCV2ORF1设计1对特异性引物,建立了SYBRGreenⅠReal-timePCR检测方法。该方法灵敏度可达10~100拷贝/μL,比常规PCR检测方法灵敏度高10倍,而与猪繁殖和呼吸综合征病毒(PRRSV)、猪伪狂犬病毒(PRV)、猪瘟病毒(CSFV)以及猪细小病毒(PPV)没有交叉反应,利用该方法从50份临床病料中检测出43份阳性PCV2病毒,阳性检出率为94%。与常规PCR比较结果显示,该方法具有较高的灵敏度和特异性,能够快速检测和监控PCV2的感染流行。

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