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小麦MITE扩增体系的建立

DOI: 10.7668/hbnxb.2014.02.021, PP. 110-115

Keywords: 小麦叶锈病,抗病基因,MITE,分子标记

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Abstract:

以Thatcher及23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系为材料,开展了MITE扩增体系的建立研究,获得了适合小麦MITE分子标记的扩增体系及不同引物的退火温度,所用56对引物有27对获得稳定扩增。

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