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土壤中黄瓜棒孢叶斑病病原菌实时荧光定量PCR检测技术研究

DOI: 10.7668/hbnxb.2014.02.013, PP. 71-74

Keywords: 多主棒孢,实时荧光定量PCR,土壤检测

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Abstract:

筛选到应用于多主棒孢实时荧光定量PCR检测的特异性引物CIR5/CIF5,并建立了土壤中多主棒孢的实时荧光定量PCR检测技术。引物CIR5/CIF5能够从多主棒孢基因组DNA中特异性扩增出一条259bp的片段,常规PCR检测中灵敏度可达10fg/μL。利用实时荧光定量PCR检测模拟带菌土壤中的带菌量,结果证明该技术对土壤中多主棒孢的检测下限为1个孢子/g土壤,可以快速、准确地定量土壤中病原菌的孢子数目,为栽培前土壤中病原菌的监测预报提供有效的技术手段。

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