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生肌因子Myogenin和Myf5真核双表达载体的构建及效果检测

DOI: 10.7668/hbnxb.2014.02.003, PP. 13-17

Keywords: 生肌过程,双表达载体,生肌因子

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Abstract:

为研究生肌因子如Myogenin和Myf5在调控肌特异基因表达时的作用机制,将这2个因子共转染非肌细胞,为确保转染效果,依次将这2个因子的编码序列克隆在双表达载体pVITRO2上。测序结果显示,克隆到载体的myogenin和Myf5编码区序列正确;初步的功能检测表明,它们都能有效地激活各自的靶基因。

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