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猪繁殖与呼吸综合征病毒变异株的分离鉴定及遗传变异分析

DOI: 10.7668/hbnxb.2014.01.042, PP. 232-238

Keywords: 猪繁殖与呼吸综合征病毒,分离与鉴定,遗传变异

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Abstract:

从江苏某猪繁殖与呼吸综合征(PRRS)发病猪场采集的血清样本中分离到1株猪繁殖与呼吸综合征病毒(PRRSV)毒株,对该毒株的生物学特性进行了鉴定,并对其Nsp2和ORF5序列进行测定和序列比对分析。利用细胞传代的方法对PRRSV检测阳性,PPV和PRV检测阴性的猪血清进行病毒分离。通过RT-PCR、CPE和IFA对PRRSV分离毒株进行鉴定,并测定其TCID50。测序获得Nsp2和ORF5基因,并利用DNAStar软件进行序列同源性和进化分析。成功分离获得1株PRRSV毒株,该毒株能够在Marc-145细胞上增殖并产生细胞病变,免疫荧光检测可观察到特异性荧光,TCID50为105.5。Nsp2序列分析表明,该分离株具有HP-PRRSV30个氨基酸缺失的基因特征,同时495~516位也存在缺失。分离株ORF5基因与其他参考毒株氨基酸同源性为57.1%~99.5%,与NJ-1106的同源性最高,为99.5%。系统进化树分析表明,该分离株属于北美型,与WUH4、NJ-1106等HP-PRRSV毒株属于同一分支。本试验分离得到一株HP-PRRSV变异毒株,为分析我国PRRSV的遗传变异和防控奠定了基础。

References

[1]  1 Zimmerman J J, Yoon K J,Wills RW,et al. General over-view of PRRSV:a perspecaive from the United States[J]. Microbio1,1997,55(1-4):187-196
[2]  2 Rossow K D. Porcine reproducaive and respiratory syn-drome[J]. Vet Pathol,1998, 35:1-20
[3]  3 Kelfaver K K. Reproducaive failure of unknown etiology[J]. Am Assoc Swine Praca News,1989,1(2):1-9
[4]  4 Nelsen C J, Murtaugh M P, Faaberg K S. Porcine repro-ducaive and respiratory syndrome virus comparison:diver-gent evolution on two continents[J]J Virol, 1999,73(1):270-80.
[5]  5 Meulenberg J J,de Meijer E J,Moormann R J. Subgenom-ic RNAs of Lelystad virus。ontain a conserved leader-body juncaion sequence[J].J Gen Viro1,1993,74(8):1697-1701
[6]  6 Stadejek T, Oleksiewicz M B,Potapc:huk D, et al. Porcine reproducaive and respiratory syndrome virus strains of ex-ceptional diversity in eastern Europe support the definition of new genetic: subtypes [J]. J Gen Virol,2006, 87 (Pt7): 1835-1841
[7]  13 安同庆,田志军,李冉,等我国高致病性猪蓝耳病病毒的演化分析[J]中国兽医杂志,2011,47(4);3-6
[8]  14 王小敏,何孔旺,张文文,等2008-2010年中国华东地区PRRSV ORFS基因遗传变异分析[J]中国农业科学,2011,44(18):3886-3894
[9]  15 Den Boon J A,Faaherg K S,Meulenherg J J, et al. Pro-cessing and evolution of the N-terminal region of the ar-terivirus replicase ORFla protein:identification of twoPapainlike cysteine proteases[J]. Virol, 1995,69(7):4500-4505
[10]  16 Li Y,Wang X,Bo K,et al. Emergence of a highly patho-genic porcine reproducaive and respiratory syndrome vi-rus in the Mid-Eastern region of China[J]. Vet J,2007,174(3);577-584
[11]  17 Zhou L,Zhang J,Zeng J,et al. The 30-amino-acid deletionin the Nsp2 of high1y pathogenic: porcine reproducaive and respiratory syndrome virus emerging in China is not relat-ed to its virulence[J]I Virol, 2009,83(10):5156-5157
[12]  7 Collins J E,Benfield D A, Christianson WT, et al. Isola-tion of swine infertility and respiratory syndrome virus (i-solute ATCC VR-2332) in North America and experimen-tal reproducaion of the disease in gnotobiotic pigs[J].J Vet Diagn,1992,4(2):117-126
[13]  8 郭宝清,陈章水,刘文兴,等从疑似PRRSV感染的流 产胎儿分离猪生殖和呼吸综合征病毒(PRRSV)的研 究[J]中国畜禽传染病,1996,87(2);1 -5
[14]  9 宋凌百,文英会,崔保安,等猪繁殖与呼吸综合征在 我国的流行动态[J]上海畜牧兽医通讯,2005 (2); 43-45
[15]  10 Tian K G, Yu X L, Zhao T Z,et al. Emergence of fatal PRRSV variants:unparalleled outbreaks of atypical PRRS in China and molecular dissecaion of the unique hallmark[J]. P1oS ONE,2007,2(6);526-539
[16]  11 Tong G Z, Zhou Y J, Hao X F, et al. Highly pathogenic porcine reproducaive and respiratory syndrome, China [J].Emerging Infecioust Diseases,2007,13(9):1434-1436
[17]  12 Zhou L, Yang H C. Porcine reproducaive and respiratory syndrome in China[J]. Virus Res,2010,154(1-2):31-37
[18]  18 Zhou Y J, Yu H, Tian Z J, et al. Genetic: diversity of theORFS gene of porcine reproducaive and respiratory syn-drome virus isolates in China from 2006 to 2008[J]Virus Res,2009,144(1-2):136-144
[19]  19 Yin G, Gan L, Shu X, et al. Genetic: diversity of theORFS gene of porcine reproducaive and respiratory syn-drome virus isolates in southwest China from 2007 to2009[J]. Plos One,2012,7(3):e33756
[20]  20 Wei X,Decker J M,Wang S,et al. Antibody neutraliza-tion and escape by HIV-1[J]Nature, 2003,422(6929);307-312
[21]  21 Han J,Wang Y,Faaberg K S. Complete genome analysisof RFLP 184 isolates of porcine reproducaive and respira-tort'' syndrome virus[J]. Virus Res,2006,122(1-2):175-182.

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