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6-苄基腺嘌呤延缓水稻衰亡效应的蛋白质组学分析

DOI: 10.7668/hbnxb.2014.01.003, PP. 14-19

Keywords: 水稻,6-苄基腺嘌呤,衰亡,蛋白质组学

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Abstract:

以苗期杂交水稻威优916为试验材料,分别以蒸馏水和8mg/L6-苄基腺嘌呤溶液培养,通过对功能叶片蛋白质组差异表达分析,以揭示养分胁迫下6-苄基腺嘌呤延缓水稻衰亡的效应及其机理。叶片全蛋白质经双向电泳分离,获得了22个响应6-苄基腺嘌呤处理的蛋白质,经质谱分析,其中15个功能得到鉴定,涉及光合作用、呼吸作用、激素代谢及逆境反应等多个代谢途径。分析结果显示,养分胁迫下,6-BA诱导了叶片中光合蛋白质、呼吸代谢相关蛋白质的表达量上调,减少衰亡发生密切相关蛋白质的积累,从而提高叶片光合能力,促进呼吸,满足植株生长对物质及能量的需求,延缓植株衰亡。

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