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转酵母gshI基因拟南芥的抗旱生理机制分析

DOI: 10.7668/hbnxb.2014.01.002, PP. 7-13

Keywords: gshI基因,拟南芥,转基因,耐旱性,生理生化指标

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Abstract:

以转gshⅠ基因拟南芥为试验材料,测定干旱胁迫下转基因植株的gsh含量、MDA含量和相对电导率,同时测定谷胱甘肽还原酶(GR)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,研究酵母gshI基因的功能,分析转基因拟南芥的抗旱生理机制。结果表明:干旱胁迫下,转基因植株和对照的gsh含量都有所增加,但转基因植株增加更多,干旱诱导了对照和转基因植株gsh的合成,gshI基因的表达提高了转基因植株中合成gsh的水平。转基因植株中GR活性比对照的高得多,提高了gsh/GSSG比率,使氧化态gsh得到还原,干旱启动了gsh的再生系统。转基因植株叶片相对含水量变化不明显(与对照相比),但MDA含量和相对电导率增加幅度均低于对照植株,转基因植株提高了膜系统的稳定性(抗氧化作用)。干旱胁迫下,对照和转基因植株SOD和CAT活性均下降,但没有明显差异,表明gsh与SOD、CAT对活性氧的清除机制不同。

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