全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

四川白鹅ENO1基因特征及其在HPG轴组织中发育性表达的研究

DOI: 10.7668/hbnxb.2014.01.018, PP. 93-97

Keywords: 四川白鹅,ENO1,生物信息学分析,下丘脑-垂体-性腺轴,基因表达

Full-Text   Cite this paper   Add to My Lib

Abstract:

旨为研究鹅ENO1基因分子特征,阐明不同繁殖期鹅下丘脑、垂体和卵巢组织ENO1表达规律。应用生物信息学技术分析并预测四川白鹅ENO1结构和功能,实时荧光定量PCR技术检测产蛋前期(6月龄)和产蛋期(8月龄)鹅下丘脑、垂体和卵巢组织ENO1的表达量。结果表明,四川白鹅ENO1是内源性蛋白质,含3个N-糖基化位点和16个磷酸化位点,二级结构以α-螺旋和无规则卷曲为主,主要分布于细胞质(73.9%)和细胞核(13.0%)。四川白鹅ENO1的4个Mg2+结合位点和1个烯醇化酶标签进化保守,但是其纤溶酶原受体结构域和MBP-1结构域分别与人氨基酸序列存在4处(422Arg→Lys、432Arg→Leu、433Ile→Ala和434Asn→Lys)和5处变异(124Ala→Val、141Pro→Ser、176Asp→Ala、177Thr→Asn和223Ala→Gly)。产蛋期鹅下丘脑、垂体和卵巢组织ENO1表达量分别是产蛋前期的1.80(P<0.05),1.64(P<0.05)和3.05倍(P<0.05)。四川白鹅ENO1是多功能的、进化保守的内源性蛋白质,ENO1可通过调节HPG轴功能来参与调控鹅的繁殖功能。

References

[1]  17 Redlitz A, Fowler B J, Plow E F, et al. The role of anenolase-related molecule in plasminogen binding to cells[J]. European Journal of Biochemistry /FEBS, 1995,227(1-2);407-415
[2]  18 Andronicos N M,Chen E I, Baik N,et al. Proteomic、-based discovery of a novel, strucaurally unique, and de-velopmentally regulated plasminogen receptor, Plg-RKT,a major regulator of ce11 surface plasminogen acaivation[J]. B1ood,2010,115(7):1319-1330
[3]  19 Diaz-Ramos A, Roig-Borrellas A, Garcia-Melero A, et alAlpha-Enolase, a multifuncaional protein:its role onpathophysiological situations[J]. Journal of Biomedicine&Biotechnology,2012,2012: 156795
[4]  20 Gillott D J, Eldib A, Iammarrone E,et al. Glyc:olytic: en-zyme expression in human granulosa cells[J]Fertilityand Sterility, 2008, 90 (4 Suppl):1405-1410
[5]  21 Buttedield D A, Lange M L. Multifuncaional roles of eno-lase in Alzheimer''s disease brain;beyond altered glucosemetabolism[J]Journal of Neurochemistry, 2009,111(4) :915-933
[6]  1 Kogure K,Nakamura K,Ikeda S,et al. Glucose-regulatedprotein,78-kilodalton is a modulator of luteinizing hor-mone receptor expression in luteinizing granulosa cells inrats[J]. Biology of Reproducaion,2013,88 (1):8
[7]  2 Herrick J R, Brad A M, Krisher R L. Chemical manipula-tion of glucose metabolism in porcine oocytes:elfecas onnucaear and cytoplasmic maturation in vitro[J]Repro-ducaion,2006,131(2);289-298
[8]  3 Sutton-Mcdowall M L,Gik:hrist R B,Thompson J G. Thepivotal role of glucose metabolism in determining oocytedevelopmental competence[J]Reproducaion,2010,139(4);685-695
[9]  4 Kaneko N,Gotoh A, Okamura N,et al. Potential tumormarkers of renal cell carcinoma:alpha-enolase for postop-erative follow up,and galecain-1 and galecain-3 for prima-ry detecaion [J]. International Journal of Urology:OfficialJournal of the Japanese Urological Association,2013,20(5);530-535
[10]  5 Godier A, Hunt B J. Plasminogen receptors and their rolein the pathogenesis of inllammatory,autoimmune and ma-lignant disease[J]. Journal of Thrombosis and Haemosta-sis : JTH,2013,11(1)}26-34
[11]  6 Sedoris K C,Thomas S D,Miller D M. Hypoxia inducesDifferential translation of enolase/MBP-1[J]. BMC Canc-er,2010,10:157
[12]  7 Diaz-Ramos A, Roig-Borrellas A, Garcia-Melero A, et al Requirement of plasminogen binding to its cell-surface re-ceptor alpha-enolase for efficient regeneration of normal and dystrophic: skeletal muscae[J]P1oS One, 2012,7 (12):e50477
[13]  8 Kang B, Guo J R, Yang H M, et al. Differential expression profiling of ovarian genes in prelaying and laying geese [J]. Poultry Science,2009, 88 (9):1975-1983
[14]  9 Pancholi V. Multifuncaional alpha-enolase:its role in dis-eases[J]. Cellular and Molecular Life Sciences:CMLS, 2001,58(7):902-920
[15]  10 Kim R Y, Lietman T, Piatigorsky J, et al. Strucaure and expression of the duck alpha-enolase/tau-crystallin-en-coding gene[J]. Gene,1991,103(2):193-200
[16]  11 Cannon J, Staunton L, O''connell K, et al. Phosphopro-teomic analysis of aged skeletal muscae [J]. Internation-al Journal of Molecular Medicine,2008,22(1):33-42.
[17]  12 Tomaino B, Cappello P, Capello M, et al. Circulating au-toantibodies to phosphorylated alpha-enolase are a hall-mark of pancreatic cancer [J]. Journal of Proteome Re-search,2011,l0(1):105-112
[18]  13 Owen J B,Di Domenico F,Sultana R,et al. Proteomic、-determined dilferences in the concanavalin-A-fracaiona-ted proteome of hippocampus and inferior parietal lobulein subjecas with Alzheimer''s disease and mild cognitiveimpairment; implications for progression of AD [J]. Jour-nal of Proteome Research,2009, 8 (2); 471-482
[19]  14 Kang H J, Jung S K, Kim S J, et al. Strucaure of humanalpha-enolase(hEN01),a multifuncaional glycolytic en-zyme[J]Acaa Crystallographic:a Secaion D,BiologicalCrystallography, 2008, 64 (Pt 6); 651-657
[20]  15 Lopez-Villar E,Monteoliva L,Larsen M R,et al. Geneticand proteomic evidences support the localization of yeastenolase in the ce11 surface [J]. Proteomic、,2006,6(Sup-p1 1):107-118
[21]  16 cFen S, Arcuri D,Piddini E,et al. E、OI gene producabinds to the c-myc promoter and acas as a transcriptionalrepressor; relationship with Myc promoter-binding pro-tein 1(MBP-1)[J]. FEBS Letters,2000,473(1);47-52

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133