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利用VIGS技术分析野生茄Ve基因的抗黄萎病功能

DOI: 10.7668/hbnxb.2014.01.039, PP. 217-221

Keywords: 野生茄,VIGS,黄萎病,Ve基因

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Abstract:

黄萎病是影响茄子生产的一种重要土传性病害,挖掘利用抗病基因对茄子抗黄萎病育种具有重要的意义。Ve类基因是已知的植物抗黄萎病基因之一。采用同源法从抗黄萎病野生茄中分离Ve类基因的同源序列,构建重组载体TRV2-SlVe,经农杆菌介导侵染植株并接种黄萎病病菌,结合表型观察和半定量RT-PCR技术对结果进行了分析。结果发现,TRV2-SlVe成功地抑制了野生茄中Ve基因的表达,接种植株表现出明显的黄萎病症状。以上结果表明,野生茄中的Ve类基因具有抗黄萎病的特性。

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