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硫硒钨对钼酸盐吸收及钼酸盐转运子的影响

DOI: 10.7668/hbnxb.2015.05.036, PP. 232-238

Keywords: ,同源基因,硫酸盐,硒酸盐,钨酸盐,甘蓝型油菜

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Abstract:

为研究硫酸盐、硒酸盐和钨酸盐对甘蓝型油菜钼吸收及钼转运子的影响,通过溶液培养试验,研究了不同竞争离子对甘蓝型油菜生长及钼含量的影响及对预测钼酸盐转运基因表达的影响。结果表明,缺硫处理显著降低单株干物质重和地上部硫含量,同时提高了地上部和地下部钼含量。施硒处理对钼含量影响不大,施钨处理显著降低了钼含量,说明钨对钼吸收具有更高的抑制作用。同时发现,缺钼处理对硫含量没有显著影响。另外,通过测定拟南芥MOT1和MOT2同源基因在甘蓝型油菜中表达可以看出,相对于Bol001439和Bal035189,基因Bra007781、Bol020963和Bra011159整体在地上部和地下部都具有较高的表达水平。Bra007781、Bol020963和Bra011159主要在地上部表达。Bol001439和Bal035189具有较低的表达,但是缺钼显著影响了根部表达。相对于+Se,+W处理对BnMOT1s表达量具有较高的影响,对BnMOT2s具有低的影响。推测Bra007781、Bol001439是钼高亲和转运子,Bol020963和Bra011159是钼低亲和转运子,Bra035189不参与钼转运,而ST5;1是一个硫酸盐和钼酸盐共转运子。通过竞争离子对油菜钼营养进行研究且首次对甘蓝型油菜上钼酸盐转运基因进行推测。

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