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无机营养N,P,Ca和K对黑斑病菌侵染甘蓝幼苗的影响

DOI: 10.3321/j.issn:1000-7091.2006.05.027, PP. 113-117

Keywords: 甘蓝,黑斑病,营养液,症状表达

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Abstract:

将甘蓝幼苗分别培养在缺N(oN)、缺P(oP)、缺Ca(oCa)或缺K(oK)的不完全营养液中,培养56d后分别于离体(Seg)和非离体(Int)叶片上接种黑斑病菌,以N,P,Ca和K营养素齐全的全营养液为对照,通过调查不同营养液处理后甘蓝叶片黑斑病的病斑大小,探讨N,P,Ca和K等无机营养素对黑斑病菌侵染甘蓝幼苗的影响。试验分2部分完成:第1部分缺N,缺P和缺Ca3个处理同时进行,试验结果表明,不同营养液培养的甘蓝叶片黑斑病病斑大小有显著差异,顺序为对照>缺P>缺Ca>缺N处理;而在对照、缺P和缺Ca处理中,同一处理的非离体叶片病斑明显大于离体叶片病斑,缺N处理情况则相反。第2部分缺K处理的试验中,接种后5d的病斑明显小于对照,非离体叶片与离体叶片接种后的病斑分别比对照小53%和14%;而缺K处理的离体叶片病斑显著比非离体叶片大(37%),并且病情随着接种后时间的延长而加重,在接种22d后植株叶片和离体叶片的病斑大小趋于平衡。相关性分析表明,接种后5d甘蓝幼苗活体组织和衰老组织的症状表达没有明显的相关性,但从试验2接种后22d以及整个试验所有数据的结果分析,两者之间存在一定的相关性,相关系数达r=0.61。

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