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氯霉素单克隆抗体免疫金标快速检测试纸的研制及性能测定

DOI: 10.3321/j.issn:1000-7091.2006.05.030, PP. 127-131

Keywords: 氯霉素,单克隆抗体,胶体金免疫层析试验,快速检测试纸

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Abstract:

以分泌抗氯霉素单克隆抗体(CAPmAb)杂交瘤细胞株的建立为基础,应用胶体金免疫层析试验(GICA)成功研制出CAP残留快速检测试纸(CAP_Strip),并对其性能进行了测定。结果表明,CAP_Strip的标准曲线呈典型的S型,符合4参数logit曲线拟合,目测检测限为0.5μg/L;与氯霉素琥珀酸钠的交叉反应为160%,与其他酰胺醇类和抗菌药物无交叉反应;其敏感性与竞争ELISA试剂盒相当,符合率为100%;10min内显示结果,具有快速、敏感、特异、简便等特点,适合CAP残留快速检测的推广应用。

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