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蒙古马肌生成抑制基因(MSTN)的克隆及序列分析

DOI: 10.3321/j.issn:1000-7091.2006.05.012, PP. 45-49

Keywords: 蒙古马,MSTN,基因,克隆,测序

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Abstract:

肌生成抑制基因(MSTN)在发育和成熟的骨骼肌中特异表达,并对肌肉发育和再生具有负调控作用。依据马肌生成抑制素(MSTN)编码序列设计引物,以蒙古马基因组DNA为模板,利用PCR技术,扩增出MSTN基因的几个片段后,测序并按顺序拼接,得到DNA全序列。序列分析结果表明,MSTN基因由2个内含子和3个外显子组成,碱基数量分别为第一外显子373bp、第二外显子374bp、第三外显子381bp;第一内含子1833bp、第二内含子2018bp,共有4979个碱基。该序列首次登录到GenBank,登录号为AY840554。

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