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含苏云金芽孢杆菌aiiA基因的重组荧光假单胞菌的构建及对软腐病的抗病活性

DOI: 10.3321/j.issn:1000-7091.2006.04.004, PP. 13-16

Keywords: 荧光假单胞菌工程菌,群体感应,aiiA基因,胡萝卜软腐欧文氏杆菌,细菌病害防治

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Abstract:

aiiA基因编码的aiiA蛋白能降解细菌群体感应系统中的信号分子N_酰基高丝氨酸内酯AHLs,从而减弱致病菌对植物的危害。本文将aiiA基因转入到植物根际促生细菌荧光假单胞菌P303中,构建成防治植物细菌和真菌病害的工程菌P303-ss10。通过PCR和RT-PCR鉴定,均获得0.8kb的目的片断,表明aiiA基因已经导入P303中。室内平板抑菌试验表明,该工程菌保留了出发菌株对多种植物病原真菌的抑制作用;室内离体试验表明该工程菌对马铃薯软腐病和大白菜软腐病都有一定的抑制作用。

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