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内标18SrRNA和-βactinmRNA比较:肉鸡十二指肠SGLT1mRNA的发育规律

DOI: 10.3321/j.issn:1000-7091.2006.03.026, PP. 112-116

Keywords: 18SrRNA,-&beta,actinmRNA,内标,SGLT1mRNA,肉鸡

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Abstract:

β-actinmRNA和18SrRNA因其在相同试验条件下的稳定表达,而被广泛用作内标基因来确定目的基因的表达量。试验分别使用这2种内标基因,用相对定量RT-O36d)十二指肠钠葡萄糖共转运载体1(Sodiumglucosecotransporter1,SGLT1)的发育规律进行了分析研究,比较不同内标基因对SGLT1mRNA表达水平检测结果的影响,以确定两者在一致试验条件下表达的相似度。结果表明,分别以-βactinmRNA和18SrRNA作为内标,SGLT1mRNA表达发育规律完全一致,均表现为2~30d不断升高,44d下降,58d回升;2d和44d的SGLT1mRNA丰度显著低于16d(P<0.05)和30d(P<0.05),58d的SGLT1mRNA表达水平显著低于30d(P<0.05)。提示这2种管家基因均可以作为内标用于肉鸡肠道转运载体基因mRNA发育规律的相对定量研究。

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