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小麦抗叶锈病基因Lr35的RGA分析

DOI: 10.7668/hbnxb.2008.06.011, PP. 50-53

Keywords: 小麦叶锈病,抗病基因,Lr35,抗病基因类似序列

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Abstract:

根据已知植物抗病基因的NBS,LRR等保守结构域设计引物,对小麦全套抗叶锈病近等基因系材料进行RGA分析。引物对Pto-kin1IN/XLRR-INV1从近等基因系TcLr35中扩增获得一条77bp的特异性片段。序列分析表明,该片段与小麦基因片段TriticumurartucloneBAC210J2,TriticummonococcumDV92的BAC克隆231A16等具有较高同源性,为Lr35的克隆奠定了基础。

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