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玉米蛋白激酶基因ZmPti1-1的cDNA克隆及其表达特性

DOI: 10.7668/hbnxb.2008.06.008, PP. 36-40

Keywords: 玉米,Pti1,生物胁迫,非生物胁迫

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Abstract:

应用电子克隆的方法获得了玉米的一个Pti1(Pto-interacting1)同源基因的全长cDNA,命名为ZmPti1-1(GenBank接受号为EF1803)。推断ZmPti1-1蛋白含有362个氨基酸,分子量为39.00kDa,pI为8.14,包含蛋白激酶催化结构域保守的11个亚结构域。在水杨酸(Salicylicacid,SA)、甘露醇、氯化钠、脱落酸(Abscisicacid,ABA)和4℃低温的诱导下,ZmPti1-1在玉米幼苗叶片中的表达量明显上调。同时,在玉米不同发育时期、不同组织器官内,ZmPti1-1的表达水平也有很大差异,在子房中表达量最高,在雄蕊中检测不到表达。试验结果表明,ZmPti1-1是一个玉米类Pti1基因,响应生物胁迫和非生物胁迫的诱导。

References

[1]  Tang X,Frederick R D,Zhou J,et al.Initiation of plant disease resistance by physical interaction of AvrPto and Pto kinase[J].Science,1996,274:2060-2063.
[2]  Scofield S R,Tobias C M,Rathjen J P,et al.Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato[J].Science,1996,274:2063-2065.
[3]  Martin G B,Brommonschenkel S,Chunwongse L,et al.Map-based cloning of a protein kinase gene conferring disease in tomato[J].Science,1993b,262;1432-1436.
[4]  Tian A G,Luo G Z,Wang Y J.Isolation and characterization of a Pti1 homologue from soybean[J].J Exp Bot,2004,396:535-537.
[5]  Anthony R G,Khan S,Costa J,et al.The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidatice stress signaling pathways downstream of PDK1 and OXI1[J].Journal of Biology Chemistry,2006,281;37536-37546.
[6]  Takahashi A,Agrawal K G,Yamazaki M,et al.Rice Pti1a negatively regulates RAR1-dependenr defense responses[J].The Plant Cell,2007,19:2940-2951.
[7]  Zou H W,Wu Z Y,Yang Q,et al.Gene expression analyses of ZmPti1,encoding a maize Pti-like kinase,suggest a role in stress signaling[J].Plant Science,2006,171 (1):99-105.
[8]  Herrmann M M,Pinto Sheena,Kluth J,et al.The Pti1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte[J].BMC Plant Biology,2006,6:1-22.
[9]  Hanks S K,Quinn A M,Hunter T.The protein kinase family:conserved features and deduced phylogeny of the catalytic domains[J].Science,1988,241:42-52.
[10]  Sessa G D,Ascenzo M,Martin G B.The major site of the Pti1 kinase phosphorylated by the Pto kinase is located in the activation domain and is required for Pto-Pti1 physical interaction[J].European Journal of Biochemistry,2000,267:171-178.
[11]  Thomashow M F.Plant cold accumulation:freezing tolerance genes and regulatory mechanisms.Annu.Rev.Plant Physiol[J].Plant Molecular Biology,1999,50:571-599.
[12]  Shinozaki K,Yamaguchi-Shinozaki K.Molecular responses to dehydration and low temperature:Differences and cross-talk between two stress signaling pathways[J].Current Opinion in Plant Biology,2000,3:217-223.
[13]  Keen N T.Gene-for-gene complementarity in plant-pathogen interactions[J].Annu Rev Genet,1990,24:447-463.
[14]  Dangl J L.The enigmatic avirulence genes of phytopathogenic bacteria[J].Curr Top Microbiol Immunol,1994,192:99-118.
[15]  Hammond-Kosack K E,Jones J D A.Plant disease resistance genes[J].Annu Rev Plant Physiol Plant Mol Biol,1997,48:575-607.
[16]  Hulbert S H,Webb C A,Smith S M,et al.Resistance gene complexes:evolution and utilization[J].Annu Rev Phytopathol,2001,39:285-312.
[17]  Gabriel D W,Rolfe B G.Working models of specific recognition in plant-microbe interactions[J].Annu Rev Phytopathol,1990,28:365-391.
[18]  Zhou J,Loh Y T,Bressan R A,et al.The tomato gene Ptil encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response[J].Cell,1995,83:925-935.

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