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小麦热胁迫相关蛋白Hsa32基因的克隆

DOI: 10.7668/hbnxb.2008.06.001, PP. 1-5

Keywords: 小麦,热激蛋白,Hsa32,克隆

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Abstract:

热胁迫相关蛋白Hsa32主要存在于陆生植物中,Hsa32参与植物获得性耐热性的维持。本研究以37℃热胁迫1h的小麦幼苗叶子为材料,提取总RNA,结合RT-PCR和RACE的方法克隆获得小麦Hsa32基因,该基因完整的开放阅读框全长88bp,编码294个氨基酸。编码的氨基酸与水稻中Hsa32(OsHsa32)同一性最高,达83%,与拟南芥中Hsa32(AtHsa32)有66%的同一性,与番茄中Hsa32(LeHsa32)有63%的同一性,由此将其命名为TaHsa32。Northern杂交结果表明,TaHsa32是一个诱导型热激蛋白。

References

[1]  Li G L,Chang H,Li B,et al.The roles of the atDjA2 and atDjA3 molecular chaperone proteins in improving thermotolerance of Arabidopsis thaliana seedlings[J].Plant Science,2007,173:408-416.
[2]  Liu N Y,Ko S S,Yeh K C,et al.Isolation and characterization of tomato Hsa32 encoding a novel heat-shock protein[J].Plant Sci,2006,170:976-985.
[3]  Charng Y Y,Liu H C,Liu N Y,et al.Arabidopsis Hsa32,a novel heat shock protein,is essential for acquired thermotolerance during long recovery after acclimation[J].Plant Physiol,2006,140:1297-1350.
[4]  Sambrook J,Russell D W.Molecular cloning:a laboratory mannual[M].3rd ed.Cold Spring Harbor Laborotry Press,2001:150-153.
[5]  Lindquist S.The heat-shock response[J].Ann Rev Biochem,1986,55:1151-1191.
[6]  Georgopoulos C,Welch W J.Role of the major heat shock proteins as molecular chaperones[J].Annu Rev Cell Biol,1993,9:601-634.
[7]  Lee G J,Vierling E.A small heat shock protein cooperates with heat shock protein 70 systems to reactivate a heat-denatured protein[J].Plant Physiol,2000,122:189-198.
[8]  Hartl F U,Hayer-Hartl M.Molecular chaperones in the cytosol:from nascent chain to folded protein[J].Science,2002,295:1852-1858.
[9]  Waters E R,Lee G J,Vierling E.Evolution,structure and function of the small heat shock proteins in plants[J].J Biol Chem,1996,47:325-338.
[10]  Nover L,Bharti K,Doring P,et al.Arabidopsis and the heat stress transcription factor world:how many heat stress transcription factors do we need[J].Cell Stress Chaperones,2001,6:177-189.
[11]  Lohmann C,Eggers-Schumacher G,Wunderlich M,et al.Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis[J].Mol Gen Genomics,2004,271:11-21.
[12]  Busch W,Wunderlich M,Schoffl F.Identification of novel heat shock factor-dependent genes and biochemical pathways in Arabidopsis thaliana[J].Plant J,2008,41:1-14.
[13]  Schramm F,Larkindale J,Kiehlmann E,et al.A cascade of transcription factor DREB2A and heat stress transcription factor HsfA3 regulates the heat stress response of Arabidopsis[J].Plant J,2008,53:264-274.
[14]  Yoshida T,Sakuma Y,Todaka D,et al.Functional analysis of an Arabidopsis heat-shock transcription factor HsfA3 in the transcriptional cascade downstream of the DREB2A stress-regulatory system[J].Biochemical and Biophysical Res Communications,2008,368:515-521.
[15]  Charng Y Y,Liu H C,Liu N Y,et al.A heat-inducible transcription factor,HsfA2,is required for extension of acquired thermotolerance in Arabidopsis[J].Plant Physiol,2007,143:251-262.
[16]  Queitsch C,Hong S W,Vierling E,et al.Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis[J].Plant Cell,2000,12:479-492.
[17]  Su P H,Li H M.Arabidopsis stromal 70-kDa heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds[J].Plant Physiol,2008,146:1231-1241.
[18]  Dafny-Yelin M,Tzfira T,Vainstein A,et al.Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in Arabidopsis[J].Plant Mol Biol,2008,67:363-373.

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