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千代田草莓乙烯受体Etr2基因克隆及序列分析

DOI: 10.7668/hbnxb.2008.06.010, PP. 46-49

Keywords: 草莓,乙烯受体,基因克隆,序列分析

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Abstract:

克隆了与草莓成熟有关的乙稀受体FaEtr2基因片段,为进一步研究FaEtr2基因功能并通过基因技术改善草莓贮运性能奠定基础。以千代田草莓成熟果实中分离到的基因组DNA为模板,经PCR扩增到1条约1.0kb的特异片段,将该片段克隆到pGEM-Teasyvector上经测序分析,基全长共109bp,编号39个氨基酸残基。序列分析结果表明,该序列与Chandler-Etr2的cDNA序列同源性99%、氨基酸序列同源性为98%。

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