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杂草稻nrDNAITS片段的PCR扩增条件优化及测定

DOI: 10.7668/hbnxb.2008.06.039, PP. 168-170

Keywords: 杂草稻,nrDNA,ITS,PCR扩增条件,测序

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Abstract:

对杂草稻的nrDNAITS片段的PCR扩增条件进行了优化并测定,建立的PCR最优体系为:20μL反应体系含1μL模板DNA,0.125mmol/LdNTPs,0.5μmol/L正反向引物,1UTaq酶,2.0μL10×TaqPCRBuffer;退火温度为57℃。这样的条件下充分保证了ITSPCR产物的质量和纯度要求,直接测序结果为600bp左右,与网上结果十分类似,表明结果准确可靠。这些ITS片段的系统学信息将为杂草稻的起源进化提供有力的分子水平证据。

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