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ZmPti1基因正义和RNAi表达载体的构建及其转化玉米的研究

DOI: 10.7668/hbnxb.2008.05.001, PP. 1-5

Keywords: ZmPti1基因,正义表达载体,RNAi载体,花粉管通道法,转基因植株

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Abstract:

构建了ZmPti1基因的正义表达载体和RNAi载体,以bar基因为抗性筛选标记,通过花粉管通道法将构建的两个表达载体分别转化到玉米自交系178。收获的种子播种于营养钵中,出苗后,经0.1%的草丁膦筛选得到40株草丁膦抗性植株,进一步用PCR鉴定与PCR-Southern检测得到33株转基因植株,其中15株是转化正义表达载体的转基因植株,18株是转化RNAi表达载体的转基因植株。并对转化方法和ZmPti1基因的功能进行了讨论。

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