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苏云金杆菌vip1A/vip2A基因的鉴定及其与cry1基因的联系

DOI: 10.7668/hbnxb.2008.05.029, PP. 131-135

Keywords: 二元毒素,vip1A/vip2A,vip1A/vip2A-16,PCR-RFLP,cry1Fa

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Abstract:

利用PCR方法检测vip1A/vip2A基因在野生型Bt菌株中的分布,进行基因片段的克隆和序列分析。结合cry1基因的PCR-RFLP基因型鉴定体系,分析与vip1A/vip2A基因有联系的cry1类基因。结果表明,分离保存的171株野生型Bt菌株中,有20株菌株含有vip1A/vip2A基因,分布率为11.69%。成功克隆了菌株Bt16的vip1A/vip2A基因片段,命名为vip1A/vip2A-16,GenBank登录号为EU94327.cry1基因的PCR-RFLP鉴定结果表明,该20株菌株均含有cry1Fa基因,说明vip1A/vip2A基因与cry1Fa可能具有某种联系。

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