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小麦抗叶锈病近等基因系TcLr41基因的差异表达

DOI: 10.7668/hbnxb.2008.05.027, PP. 122-126

Keywords: 小麦叶锈病,抗病基因,cDNA-AFLP,Lr41

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Abstract:

为了研究与小麦抗叶锈病相关基因的表达情况,从分子水平阐明小麦的抗病机制。以Thatcher和Thatcher为遗传背景的小麦抗叶锈病近等基因系TcLr41为材料,利用cDNA-AFLP技术,开展了与小麦抗叶锈病基因Lr41相关的差异表达基因研究。共选用180对引物组合对Lr41差异表达的基因进行了分析,获得了61对能够在小麦近等基因系TcLr41和感病对照Thatcher之间扩增出差异条带的多态性引物。获得对能够扩增出条对小麦抗叶锈病近等基因系TcLr41具有特异性条带的引物,分别是P-AC/M-CAA、P-AG/M-CAT、P-AG/M-CCC、P-CC/M-CCA、P-GA/M-CAA。将重复性好的4条差异片段进行回收、克隆、测序,经序列同源性检索发现了1条与小麦抗叶锈病基因Lr1编码蛋白同源性较高的序列,其他的3个序列功能未知,研究结果为探明TcLr41的抗病机制和进行该抗病基因的克隆奠定了基础。

References

[1]  闫红飞,杨文香,刘大群.小麦抗叶锈近等基因系TcLr38抗病相关基因片段的分离[J].植物遗传资源学报,2007,8(3):308-312.
[2]  Bachem C W B,Van der Hoeven R S,de Bruijn S M,et al.Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP:Analysis of gene expression during potato tuber development[J].Plant J,1996,9:745-753.
[3]  Money T,Reader S,Qu L J,et al.AFLP-based mRNA fingerprinting[J].Nucleic Acids Res,1996,24:2616-2617.
[4]  薛春生,肖淑芹,王国英,等.利用cDNA-AFLP技术分析玉米自交系黄早四甘蔗花叶病毒侵染后基因差异表达[J].植物病理学报,2005,35(3):229-234.
[5]  Umezawa T,Mizuno K,Fujimura T.Discrimination of genes expressed in response to the ionic or osmotic effect of salt stress in soybean with cDNA-AFLP[J].Plant Cell Environment,2002,25(12):1617-1625.
[6]  秘彩莉,张学勇,温小杰,等.利用 cDNA-AFLP 技术获得小麦耐盐性相关基因 TaVHA-C[J].中国农业科学,2006,39(9):1736-1742.
[7]  陈云芳,杨文香,闫红飞,等.小麦抗叶锈病近等基因系TcLr35基因表达差异研究[J].植物病理学报,2007,37(1):109-112.
[8]  Mao C,Yi K,Yang L,et al.Identification of aluminium-regulated genes by cDNA-AFLP in rice(Oryza sativa L.):aluminium-regulated genes for the metabolism of cell wall components[J].J Exp Bot,2004,55(394):137-143.
[9]  Samuelian S,Kleine M,Ruyter-Spira C P,et al.Cloning and functional analyses of a gene from sugar beet up-regulated upon cyst nematode infection[J].Plant Mol Biol,2004,54(1):147-156.
[10]  Eckey C,Korell M,Leib K,et al.Identification of powdery mildew-induced barley genes by cDNA-AFLP:functional assessment of an early expressed MAP kinase[J].Plant Mol Biol,2004,55(1):1-15.
[11]  Cox T S,Raupp W J,Gill B S.Leaf rust-resistance genes Lr41,Lr42 and Lr43 transferred from Triticum tauschii to common wheat[J].Crop Sci,1994,34:339-343.
[12]  Lottering J M,Botha A M,Klopper F J.AFLP and RAPD markers linked to leaf rust resistance gene Lr41 in wheat[J].South African Journal of Plant and Soil,2002,19:1-6.
[13]  Habu Y,Fukada-Tanaka S,Hisatomi Y,et al.Amplified restriction fragment length polymorphism-based mRNA finger printing using a single restriction enzyme that recognizes a 4-bp sequence[J].Biochen Biophy Res Commun,1997,234:516-521.
[14]  Fukuda T,Kido A,Kajino K.Cloning of differentially expressed genes in highly and low metastaic rat Osteosarcomas by a modified cDNA-AFLP method[J].Biochen Biophy Res Commun,1999,261(1):35-40.
[15]  张峰,宋文芹,陈瑞阳.AFLP-银染法检测植物基因组多态性[J].细胞生物学杂志,1999,21(2):98-101.

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