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病毒复制酶基因介导玉米抗矮花叶病的研究

DOI: 10.7668/hbnxb.2008.05.025, PP. 114-117

Keywords: 病毒复制酶基因,玉米,花粉介导法,人工接种,矮花叶病毒

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Abstract:

本研究将病毒复制酶基因(NIa、NIb)通过花粉介导法,转入玉米优良自交系中。对所转化的玉米后代进行了PCR检测,并对检测呈阳性的植株于次年播种于试验田,对转化的植株连续2代(T1和T2代)利用人工摩擦接种矮花叶病毒的方法进行田间抗病试验。将连续2代抗病的植株通过PCR以及PCR-SouthernBlot检测结果证明:病毒复制酶基因已成功导入玉米自交系中,结合田间抗性鉴定,获得了对玉米矮花叶病抗病性增强且农艺性状良好的玉米株系。

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