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马铃薯野生种CHS基因cDNA的克隆与表达分析

DOI: 10.7668/hbnxb.2008.05.004, PP. 17-22

Keywords: 查尔酮合酶,花色苷,cDNA克隆,表达谱,马铃薯野生种

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Abstract:

设计简并引物,用RT-PCR法从马铃薯野生种(Solanumpinnatisectum)克隆到了CHS(Chalconesynthase,查尔酮合酶)基因的全长cDNA,并用半定量RT-PCR法进行了表达分析。序列分析表明,CHS基因编码一个389个氨基酸残基的多肽,在氨基酸水平上与其他茄科植物的同源性达到89%~93%,多重比较和系统发育分析均表明该基因为CHS家族中的一个新成员;检测了CHS基因的空间表达模式,该基因在花和匍匐茎中表达量最高,在根和块茎中没有检测到,这与花和匍匐茎呈淡紫色有花色苷合成,而根和块茎呈白色无花色苷合成是相一致的;发现在块茎中CHS受光诱导表达,光照后的第3天表达量最高,而在光照前检测不到,光照前块茎为白色,随着光照时间的延长,其表层颜色因积累花色苷而变成紫红色。

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