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ahFAD2基因RNA干扰体的删除筛选标记载体构建及其遗传转化

DOI: 10.7668/hbnxb.2008.05.003, PP. 13-16

Keywords: ahFAD2,RNA干扰,删除筛选标记,载体构建

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Abstract:

将Napin启动子与含花生FAD2基因反向重复片段的RNA干扰结构连接,亚克隆至含雌激素诱导重组系统的植物表达载体pNCX相应位点中,构建完成由Napin启动子驱动的FAD2RNAi删除筛选标记表达载体pNCX-FAD2RNAi,经酶切鉴定,获得相应的启动子片段、FAD2RNAi片段及NCX-FAD2RNAi片段。利用农杆菌介导法进行遗传转化,已获得抗性丛生芽21丛。

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