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渗透胁迫下外源试剂对玉米幼苗微粒体膜Ca2+-ATPase活性的影响

DOI: 10.7668/hbnxb.2008.04.030, PP. 129-134

Keywords: 渗透胁迫,玉米,微粒体膜,2+-ATPase

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Abstract:

以钙离子鳌合剂EGTA、钙通道阻断剂Vp、钙调素拮抗剂TFP和ABA分别处理玉米幼苗,对渗透胁迫下根系和叶片质膜、液泡膜、线粒体膜以及总微膜Ca2+-ATPase活性进行了测定。结果表明,正常条件下,叶片和根系微膜系统总Ca2+-ATPase活性随光照时间延长出现一个峰值。总体比较,渗透胁迫下三种膜上Ca2+-ATPase活性相当,而且均随渗透胁迫发生不同程度的变化。就微膜系统总酶活变化而言,叶片高于根系,渗透胁迫降低叶片酶活;而渗透胁迫对根系酶活性影响不大。分解到三种膜上,渗透胁迫对叶片酶活的提高均表现为正效应,根系上,对线粒体膜和液泡膜酶活提高表现为正效应,而对质膜酶活表现为显著抑制。外源试剂处理发现,ABA和EGTA在叶片上均可逆转PEG降低总酶活的效应,且在处理6h时使得酶活性显著超过正常水平;而根系上表现不一:其中,Vp对质膜上PEG提高酶活效应抑制作用较大,且显著提高线粒体膜酶活;ABA显著提高液泡膜上酶活性;TFP处理在胁迫前期基本表现为促进作用,而6h之后大都表现为抑制效应。说明:渗透胁迫下,玉米叶片质膜、液泡膜以及线粒体膜均参与了胞质钙离子稳态的调控过程,外源试剂处理对Ca2+-ATPase具不同程度的调节作用,叶片和根系Ca2+-ATPase对外源试剂响应存在差异性。

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